کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2048826 1543464 2008 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro
چکیده انگلیسی

We describe a novel secreted protein, named hOLFML1 (human olfactomedin-like protein 1), with an olfectamine domain in its C-terminus, mainly expressed in the small intestine, liver, lung and heart. Immunohistochemical staining on human small intestine indicated that the protein localizes preferentially in the intestinal villi. Interestingly, ectopic hOLFML1 promoted proliferation of HeLa cells and increased the percentage of cells in S phase. In contrast, knock down of hOLFML1 protein expression by siRNA inhibited cell proliferation and delayed the entry of cells into S phase. Our data also revealed that hOLFML1 is N-glycosylated and its secretion is triggered by serum. Taken together, these findings suggest that hOLFML1 may play a significant role in the regulation of cell proliferation in vitro.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 582, Issues 21–22, 22 September 2008, Pages 3185–3192
نویسندگان
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