کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2050990 1074187 2007 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک دانش گیاه شناسی
پیش نمایش صفحه اول مقاله
Prion infection correlates with hypersensitivity of P2X7 nucleotide receptor in a mouse microglial cell line
چکیده انگلیسی

We recently established mouse microglial cells persistently infected with mouse-adapted scrapie ME7 (ScMG20/ME7) for in vitro study of prion pathogenesis. Here, we found that ScMG20/ME7 cells were hypersensitive to P2X7 receptor agonists, as demonstrated by sustained Ca2+ influx, membrane pore formation, cell death, and interleukin-1β release. P2X7 mRNA expression was upregulated in these cells, and also in scrapie-infected mice brains. Treatment with pentosan polysulfate eliminated the infectivity and disease-related forms of prion protein from ScMG20/ME7 cell cultures, however, hypersensitivity of P2X7 receptors remained. These results suggest that prion infections may strongly affect the P2X7 receptor system in mouse microglial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: FEBS Letters - Volume 581, Issue 16, 26 June 2007, Pages 3019–3026
نویسندگان
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