کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067577 | 1544339 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Induction of endoreduplication by a JNK inhibitor SP600125 in human lung carcinoma A 549 cells
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کلمات کلیدی
PBSJnkFBSp53ERKEndoreduplicationSP600125c-Jun N-terminal kinase - C-Jun N-terminal kinaseMAPK - MAPKMdm2 - MDM2fetal bovine serum - سرم جنین گاوPhosphate buffered saline - فسفات بافر شورmitogen-activated protein kinase - پروتئین کیناز فعال با mitogenCell cycle - چرخه سلولیextracellular-signal-regulated kinase - کیناز تنظیم شده خارج سلولی سیگنال
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Induction of endoreduplication by a JNK inhibitor SP600125 in human lung carcinoma A 549 cells Induction of endoreduplication by a JNK inhibitor SP600125 in human lung carcinoma A 549 cells](/preview/png/2067577.png)
چکیده انگلیسی
The effect of the pan c-Jun N-terminal kinase (JNK) inhibitor SP600125 on the proliferation of human lung carcinoma A549 cells has been evaluated. We have shown that SP600125 completely inhibited the proliferation of A549 cells, the cycle arrest being in G2/M phase. When cells were treated with SP600125 for >12Â h, a cell population with DNA content of 4n to 8n was detected. Moreover, the effect of SP600125 on the expression of cell cycle related proteins was an upregulation of p53 protein accompanied by an increase in its molecular mass. Prolonged SP600125 treatment downregulated p21, Bax and Mdm2 expression, but increased the level of the cellular p53-Mdm2 complex. Taken together, we show that SP600125 could induce G2/M cell cycle arrest and endoreduplication in a p21 independent manner, and that SP600125 could also post-translationally modify p53 to modify its function. Our data show that basic JNK activity plays an important role in the progression of the cell cycle at G2/M cell phase.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 12, December 2007, Pages 1501-1506
Journal: Cell Biology International - Volume 31, Issue 12, December 2007, Pages 1501-1506
نویسندگان
Yumi Miyamoto-Yamasaki, Masao Yamasaki, Hirofumi Tachibana, Koji Yamada,