کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067687 | 1077905 | 2007 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Endothelial cell and macrophage regulation of vascular smooth muscle cell calcification modulated by cholestane-3β, 5α, 6β-triol
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کلمات کلیدی
MGPPTHrPVSMCsECs - EC هاROS - ROSALP - آلکالن فسفاتازAlkaline phosphatase - آلکالین فسفاتاز یا فسفاتاز قلیاییtumor necrosis factor-α - تومور نکروز عامل αVascular smooth muscle cells - سلول های عضلانی صاف عروقیEndothelial cells - سلولهای اندوتلیالTriol - سه گانهTNF-α - فاکتور نکروز توموری آلفاMacrophages - ماکروفاژها،درشت خوارهاMatrix Gla protein - پروتئین ماتریکس GlaParathyroid hormone-related peptide - پپتید مرتبط با هورمون پاراتیروئیدVascular calcification - کلسیفیکاسیون عروقیReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The cellular and molecular mechanisms that mediate vascular calcification remain poorly understood. In our previous study, oxysterol cholestane-3β, 5α, 6β-triol (Triol) was shown to promote vascular smooth muscle cells (VSMCs) calcification. In this study, by using direct coculture, non-contact transwell coculture, and culture with conditioned media, we investigated the roles of endothelial cells (ECs) and macrophages in the regulation of VSMCs calcification in the absence or presence of Triol. In vitro calcification was induced by incubation of VSMCs with β-glycerophosphate. The results showed that ECs inhibited VSMCs calcification, as manifested by the reduction of calcium deposition in extracellular matrix. This effect of ECs on calcification was via the secreted soluble factors. Furthermore, the stimulation of ECs by Triol had no influence on ECs inhibition of calcification. On the other hand, macrophages promoted VSMCs calcification via the secreted soluble factors such as reactive oxygen species, which was further enhanced by Triol. Our results supported the roles for ECs and macrophages in vascular calcification, modulated by oxysterols in atherosclerotic plaque.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 9, September 2007, Pages 900-907
Journal: Cell Biology International - Volume 31, Issue 9, September 2007, Pages 900-907
نویسندگان
Hongmei Liu, Lan Yuan, Shanjin Xu, Kui Wang,