کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2067741 | 1077907 | 2007 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells
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کلمات کلیدی
DCFH-DAΔΨmbLFP-E2′,7′-dichlorofluorescein diacetate - 2 '، 7'-dichlorofluorescein diacetateROS - ROSApoptosis - خزان یاختهایAnticancer - ضد سرطانLactoferrin - لاکتوفرینBovine lactoferrin - لاکتوفرین گاوMitochondrial transmembrane potential - پتانسیل ترانزیتی میتوکندریTea polyphenols - پلی فنل چایReactive oxygen species - گونههای فعال اکسیژن
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوفیزیک
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells In vitro evaluation of the anticancer effect of lactoferrin and tea polyphenol combination on oral carcinoma cells](/preview/png/2067741.png)
چکیده انگلیسی
We investigated the anticancer effects of green and black tea polyphenols alone and in combination with bovine milk lactoferrin (bLF) on human tongue squamous carcinoma (CAL-27) and normal human gingival fibroblast (HGF) cells. Both green (Polyphenon-E;P-E) and black tea polyphenols (Polyphenon-B;P-B) preferentially inhibit the growth of CAL-27 cells in a dose-dependent manner. Based on the IC50 values, P-E was found to be more effective than P-B and the combination of P-E and bLF (1:2 ratio) exhibited synergistic inhibition of CAL-27 cells. Analysis of the mechanism revealed nuclear fragmentation and condensation with appearance of the Ao peak indicative of apoptosis. Furthermore, tea polyphenols transduced the apoptosis signal via generation of reactive oxygen species and decrease in the Bcl-2/Bax ratio thereby inducing mitochondrial permeability transition with consequent activation of caspase-3. Overall, the potency of cytotoxic and apoptosis inducing effects of dietary agents on CAL-27 cells was in the order P-E and bLF combination (1:2 ratio)Â >Â P-EÂ >Â P-B. These results suggest that a “designer” approach may be useful for oral cancer prevention strategies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cell Biology International - Volume 31, Issue 6, June 2007, Pages 599-608
Journal: Cell Biology International - Volume 31, Issue 6, June 2007, Pages 599-608
نویسندگان
K.V.P. Chandra Mohan, P. Gunasekaran, E. Varalakshmi, Y. Hara, S. Nagini,