کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2075326 1079328 2015 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synergistic actions of proteolytic enzymes for production of soy protein hydrolysates with antioxidant activities: An approach based on enzymes specificities
ترجمه فارسی عنوان
اقدامات هماهنگ کننده آنزیم های پروتئولیتیک برای تولید پروتئین هیدرولیز سدیم با فعالیت های آنتی اکسیدانی: روش مبتنی بر ویژگی های آنزیم
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی

The objective of this study was to investigate the enzymatic hydrolysis of soy protein isolate by screening individual and blended commercial protease preparations using a statistical mixture design. Information about the modulation of thermal inactivation of the enzyme by substrate or products of hydrolysis and the determination of synergistic effects between the enzymes on production of soy protein hydrolysates with antioxidant activities were reported. The kinetic parameters for thermal inactivation measured under reactive and non-reactive conditions indicated that product inhibition was not significant on soy protein hydrolysis using the commercial proteases Flavourzyme® 500 L, Alcalase® 2.4 L and YeastMax A. For DPPH (2,2-diphenyl-1-picrylhydrazyl) radical scavenging, the hydrolysates obtained with Flavourzyme® 500 L combined with Alcalase® 2.4 L showed the higher synergistic effect with increases of 10.9% and 13.2% in antioxidant activity as compared to the hydrolysates produced with individual enzymes. The hydrolysates obtained using the ternary mixtures of Flavourzyme® 500 L, Alcalase® 2.4 L and YeastMax A showed the highest power of inhibition of linoleic acid autoxidation. On the other hand, for reducing power assay and total antioxidant activity, the most of all interactions was antagonistic with high antioxidant activity detected for the hydrolysates produced using Flavourzyme® 500 L, individually.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 4, Issue 4, October 2015, Pages 694–702
نویسندگان
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