Thermal stability of Candida antarctica lipase B immobilized on macroporous acrylic resin particles in organic media

Lipase B from Candida antarctica (CALB) has been exploited by many researchers for synthesis of a variety of organic compounds through esterification or transesterification reactions. Among the various immobilization media reported in the literature, the porous acrylic resin utilized in Novozym-435 has been widely studied. However, the thermal stability of free CALB and immobilized CALB (Novozym-435) in organic media at elevated temperatures for prolonged periods of time is largely unexplored. Here, we present the thermal stability and swelling of the Novozyme-435 in organic solvents (toluene and diphenyl ether) where the enzyme activity was quantified using an octyl laurate assay.