کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2075498 1079337 2014 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Screening of Pseudomonas species for biosurfactant production using low-cost substrates
ترجمه فارسی عنوان
غربالگری گونه های سودوموناس برای تولید بیوسورفکتانت با استفاده از بسترهای کم هزینه
کلمات کلیدی
بیوسورفکتانت، سودوموناس، بسترهای کم هزینه ثبات، نفت، کشش سطحی
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم کشاورزی و بیولوژیک (عمومی)
چکیده انگلیسی

In the present study, four species of Pseudomonas (P. cepacia, P. acidovorans, P. picketti and P. fluorescens) were cultivated in different low-cost substrates and compared with regard to biosurfactant production. Surface tension was used as a preliminary screening standard for potential biosurfactant bacteria. The best result was obtained with P. cepacia grown in mineral medium supplemented with 2.0% corn steep liquor and 2.0% soybean waste frying oil for 144 h at 30 °C and 200 rpm. Kinematic studies on microorganism growth and biosurfactant production were performed. Surface tension of the medium was reduced to 27.57 mN/m at the end of the cultivation, yielding 5.2 g/L of isolated biosurfactant. Tests under extreme conditions of pH, temperature and NaCl indicated the stability of the biosurfactant for use in the treatment of oil-contaminated environments. The critical micelle dilution of the biosurfactant was determined and its use in the removal of motor oil from clay soil demonstrated rates greater than 80%. Washing experiments involving rocks and oily surfaces contaminated with motor oil demonstrated greater than 80% recovery rates. The crude biosurfactant was capable of dispersing approximately 80% of oil droplets in seawater and proved to be non-toxic to indigenous marine microbiota. The crude biosurfactant demonstrated no toxicity against seeds of Brassica oleracea or the microcrustacean Artemia salina employed as a bioindicator. The present findings indicate the application potential of the biosurfactant produced by P. cepacia in the oil industry as a complement to remediation processes involving contaminated soil and water.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biocatalysis and Agricultural Biotechnology - Volume 3, Issue 2, April 2014, Pages 132–139
نویسندگان
, , , , ,