Application of response surface methodology to understand the interaction of media components during pullulan production by Aureobasidium pullulans RBF-4A3

Response surface methodology was applied to understand the interactions amongst the components of a complex medium for pullulan production by a novel Aureobasidium pullulans strain using a second order quadratic model. A total of 20 experimental runs were carried out and three dimensional response surfaces were generated to study the interaction. Results indicated that the concentration of yeast extract plays a critical role and has significant influence in pullulan production compared with peptone. Understanding the effect of complex nitrogen sources in the media using statistical methods has resulted in 70.43 g/L pullulan production which is higher as compared to earlier published reports.