کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2075699 | 1079361 | 2012 | 8 صفحه PDF | دانلود رایگان |
Bacterial isolate Bacillus licheniformis P11(C) efficiently utilized agricultural residues as carbon and nitrogen sources to generate substantial amount of xylanase which exhibited activity and stability over broad pH range (5–11) and over elevated temperatures (40–100 °C), and even in presence of potential inhibitors (triton, SDS, EDTA). Purification of xylanase (4.24-fold) by ammonium sulphate precipitation and DEAE-sepharose chromatography, and analysis by SDS-PAGE and zymography showed that B. licheniformis P11(C) produced two xylanases (17.5 and 23 kDa). Furthermore, xylanase displayed exciting potential for application in fruit juice processing and bakery processes; enzyme was found to be effective in getting enhanced sugar extraction from fruit juices, clarification of fruit juices, and substantial dough-raising in bakery.
Journal: Biocatalysis and Agricultural Biotechnology - Volume 1, Issue 4, October 2012, Pages 330–337