کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2083585 1545339 2014 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and bioevaluation of nanofibers with blood-derived growth factors for dermal wound healing
ترجمه فارسی عنوان
توسعه و ارزش زیستی نانوفیلرها با عوامل تولید کننده خون برای بهبود زخم پوستی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• Smooth chitosan/poly(ethylene oxide) nanofibers were produced by electrospinning.
• The morphology of nanofibers was resistant to moist conditions.
• The bioactivity of electrospun platelet-rich plasma (PRP) was preserved.
• The nanofibers limited skin cell mobility and changed their morphology in vitro.
• Synergistic effects of nanotophography and PRP on cell growth were demonstrated.

The aim of our work was to produce a modern nanomaterial with incorporated blood-derived growth factors, produced by electrospinning, applicable in treatment of chronic wounds. Platelet-rich plasma was chosen as a natural source of growth factors. Results showed that platelet-rich plasma stimulates keratinocyte and fibroblast cell growth in vitro. Its optimal concentration in growth medium was 2% (v/v) for both types of skin cells, while higher concentrations caused alterations in cell morphology, with reduced cell mobility and proliferation. In the next step hydrophilic nanofibers loaded with platelet-rich plasma were produced from chitosan and poly(ethylene oxide), using electrospinning. The morphology of nanofibers was stable in aqueous conditions for 72 h. It was shown that electrospinning does not adversely affect the biological activity of platelet-rich plasma. The effects of nanofibers with incorporated platelet-rich plasma on cell proliferation, survival, morphology and mobility were examined. Nanofibers limited cell mobility, changed morphology and stimulated cell proliferation. Despite of the small amount of blood-derived growth factors introduced in cell culture via platelet-rich plasma-loaded nanofibers, such nanofibrillar support significantly induced cell proliferation, indicating synergistic effect of nanotopography and incorporated growth factors. The overall results confirm favorable in vitro properties of produced nanofibers, indicating their high potential as a nanomaterial suitable for delivery of platelet-rich plasma in wound healing applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: European Journal of Pharmaceutics and Biopharmaceutics - Volume 88, Issue 1, September 2014, Pages 64–74
نویسندگان
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