An antioxidant regenerating system for continuous quenching of free radicals in chronic wounds

A novel antioxidant regenerating system consisting of cellobiose dehydrogenase (CDH), cellobiose, and phenolic antioxidants with potential application for continuous quenching of free radical species in chronic wounds was developed. This antioxidant regenerating system, continuously quenched in situ produced NO, O2- and OH radicals and the produced oxidized phenolic antioxidants were regenerated back to their original parent compounds by CDH using cellobiose as electron donor. This system therefore prevented the accumulation of oxidized phenolic antioxidants. Interestingly, this study also challenges the relevance of using total antioxidant capacities values of plant crude extracts obtained using biologically none relevant radical species like (2,2-diphenyl-1-picrylhydrazyl (DPPH)), Trolox Equivalent Antioxidant Capacity (TEAC), etc. when applied as medicinal remedies. This is because methoxylated phenolic antioxidants like sinapic acid, ferulic acid; 2,6-dimethoxyphenol readily donate their electrons to these radicals (DPPH, TEAC, etc.), thereby greatly influencing the total antioxidant values although this study showed that they are not at all effective in quenching O2- radicals and again are not the most effective quenchers of NO and OH radicals as demonstrated during this study.

The exudates from the chronic wound are absorbed by the hydrogel. This activates the enzyme cellobiose dehydrogenase and also induces the diffusion of cellobiose into the layer containing CDH. The CDH then regenerate phenolic antioxidants after they quench radicals in the chronic wound. This guarantees a continuos supply of antioxidants and continuos quenching of free radicals.Figure optionsDownload high-quality image (52 K)Download as PowerPoint slide