کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088020 1545682 2015 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of mesenchymal stromal cells on human myeloid dendritic cell differentiation and maturation in a humanized mouse model
ترجمه فارسی عنوان
اثرات سلول های استرومای مزانشیمی بر تمایز و بلوغ سلول های دندریتیک میلوئید انسان در یک مدل موس موسوم به انسان
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
چکیده انگلیسی


• Establish a humanized mouse model using cord blood
• Differentiation of human dendritic cells from humanized mouse bone marrow
• Immunoregulatory effects of mesenchymal stromal cells on human dendritic cell maturation

Mesenchymal stromal cells (MSCs) have shown promise as cellular therapy in allogeneic transplantation, although the precise mechanisms underlying their benefit in clinical trials are difficult to study. We previously demonstrated that MSCs exert immunoregulatory effects in mouse bone marrow-derived dendritic cell (DC) culture. Since mouse studies do not reliably reproduce human events, we used a humanized mouse model to study the immunomodulatory effects of human MSCs on human DC immunobiology. Humanized mice were established by injection of cord blood CD34+ cells into NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NOD scid gamma, NSG) mice. Human cells were detected in the mouse bone marrow, blood, and spleen 12 weeks after transplantation. Human DCs were differentiated from humanized mouse bone marrow cells during human MSC co-culture. MSCs inhibited DC differentiation and kept DCs in an immature state as demonstrated by phenotype and function. In conclusion, humanized mouse models represent a useful method to study the function of human MSCs on human DC immunobiology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 427, December 2015, Pages 100–104
نویسندگان
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