کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2088219 1545703 2014 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Gold nanoparticle-based immuno-PCR for detection of tau protein in cerebrospinal fluid
چکیده انگلیسی

Tau protein in cerebrospinal fluid (CSF) is an important biomarker of Alzheimer's disease and some other brain diseases. Enzyme-linked immunosorbent assays (ELISAs) have been mostly used for quantification of tau and other biomarkers in CSF. However, these assays do not have sufficient sensitivity and dynamic range. In this study we tested the suitability of gold nanoparticles functionalized with tau-specific monoclonal antibody and oligonucleotide template for immuno-polymerase chain reaction (Nano-iPCR) quantification of tau protein in human CSF samples and compared it with ELISA, either commercial or newly developed with tyramide signal amplification. Our data indicate that Nano-iPCR is superior in sensitivity and detection range to ELISA in tau protein detection.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 406, April 2014, Pages 137–142
نویسندگان
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