کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2089189 1080844 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development of flow-through and dip-stick immunoassays for screening of sulfonamide residues
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Development of flow-through and dip-stick immunoassays for screening of sulfonamide residues
چکیده انگلیسی

Two formats of membrane-based competitive enzyme immunoassays (flow-through and dip-stick) have been developed for the screening of sulfonamide residues in pig muscle and milk. Membrane was coated with anti-sulfonamide antibody and a sulfonamide hapten D2-horseradish peroxidase (HRP) conjugant was used as the labeled antigen for competitive assay of sulfonamides. Visual detection limits of the flow-through or dip-stick assay were 1–5 µg L− 1 or 1–10 µg L− 1 in buffer for seven sulfonamides, respectively. Assay validation was performed using samples spiked with single sulfonamide, spiked samples were tested using the developed strip assays and results were compared with those obtained by a validated high-performance liquid chromatograph (HPLC) method. Results showed that the two strip assays were correlated well with HPLC, respectively. With assay times of 5 min (flow-through) and 15 min (dip-stick), these rapid tests could offer simple, rapid and cost-effective on-site screening tools to detect sulfonamides in pig muscle(flow-through or dip-stick) or milk(only dip-stick).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 337, Issue 1, 20 August 2008, Pages 1–6
نویسندگان
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