کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2089373 1545790 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Measurement of high affinity antibodies on antigen-immunoblots
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Measurement of high affinity antibodies on antigen-immunoblots
چکیده انگلیسی

We describe a semi-quantitative method for measuring the relative affinity of antigen-specific oligoclonal IgG bands separated by isoelectric focusing followed by blotting onto antigen-coated membrane and incubation with sodium thiocyanate. When the developed blot is digitised in greyscale, densitograms can be made and peak areas calculated using ImageJ freeware. By expressing peak area as a percentage of the total area under the curve we have shown that there is a statistically significant rise in percentage of peak area for a given band which persists with increasing molarities of sodium thiocyanate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 310, Issues 1–2, 20 March 2006, Pages 62–66
نویسندگان
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