کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2089592 | 1545788 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Optimization of culture and storage conditions for an in vitro system to evaluate thymocyte phenotype and function
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کلمات کلیدی
TECgranulocyte-macrophage colony stimulating factorGM-CSFCD127FCSECDTCrenergy coupled dyePC5FITCMajor histocompability complexDMSO - DMSOinterleukin - اینترلوکینinterleukin-7 - اینترلوکین -7tumour necrosis factor-α - تومور نکروز عامل αsingle positive - تک مثبتThymocytes - تیموسیت هاdouble positive - دو مثبتDimethyl sulfoxide - دیمتیل سولفواکسیدfetal calf serum - سرم گوساله جنینThymic epithelial cells - سلول های اپیتلیال ThymicTriple negative - سه گانه منفیTNF-α - فاکتور نکروز توموری آلفاphycoerythrin - فایکوئیریدینfluorescein isothiocyanate - فلوئورسین ایسوتیوسیاناتMHC - مجموعه سازگاری بافتی اصلیT-cell receptor - گیرنده لنفوسیت T
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Studies on thymopoiesis are critical to the understanding of T-cell homeostasis as well as the host response to T-cell depletion. Various in vitro culture systems have been used in the study of thymocyte development; however it is unclear if current co-culture methods have been fully optimized. In this study in vitro suspension cultures have been re-evaluated and the optimal storage conditions for thymocytes have been established by evaluating various methods of storing/isolating thymic tissue and isolated thymocytes as well as the source of thymic epithelial cells (TEC). It was determined that thymocytes must be freshly isolated from whole thymic tissue and ideally stored at 4 °C prior to co-culture. Co-culture with either autologous or allogeneic TEC results in similar thymocyte subset distribution as well as interleukin-7 receptor-α (CD127) expression on these subsets. To evaluate the influence of the source of TEC on one aspect of thymocyte function the effect of IL-7 stimulation on the expression of CD127 was evaluated. IL-7 stimulation resulted in a downregulation of the expression of CD127 on all thymic subsets similar to that observed in circulating CD8+ T-cells. The effect of this was the same whether TEC were autologous or allogeneic. Optimizing culture techniques and facilitating the study of individual thymocyte subsets will lead to a better understanding of thymic function and development. It could also lead to therapeutic approaches that enhance immune recovery after T-cell depletion in HIV infection, bone marrow transplantation or following chemotherapy.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 312, Issues 1â2, 30 May 2006, Pages 157-166
Journal: Journal of Immunological Methods - Volume 312, Issues 1â2, 30 May 2006, Pages 157-166
نویسندگان
Charlene D. Young, Jonathan B. Angel,