Efficient transformation of Staphylococcus aureus using multi-pulse electroporation

A new multi-pulse electroporation system was evaluated to transform Staphylococcus aureus. Compared to the conventional electroporation system, it yielded high transformation efficiency to obtain more than 3.9 × 105 S. aureus RN4220 transformed cells/1 μg plasmid DNA using a single electroporation by manipulating the poring pulse and transfer pulse.