Construction of an efficient RNAi system in the cellulolytic fungus Trichoderma reesei

• An improved RNA interference method has been developed in Trichoderma reesei using convergent dual promoters.
• Three genes Cla4, Ras2 and RhoA involved in hyphal branching were successfully silenced using this RNAi system.
• This RNAi system is applicable for high throughput RNA silencing.

An improved RNA interference method was developed in Trichoderma reesei, using convergent dual promoters for efficient and high-throughput RNA silencing. This new vector allowed for the silencing of the eGFP gene and target genes to occur simultaneously, significantly facilitating the rapid screening of the transformants using eGFP as a reporter.