Strain development and optimized fermentation conditions for blood meal using Aspergillus niger and Aspergillus oryzae

• Two mutants were generated using ultra-violet mutagenesis.
• The mutants were screened on blood meal fermentation medium.
• A series of statistical methods were used to optimize the fermentation conditions.

To hydrolyze blood meal (BM) effectively, two mutants were generated using ultra-violet mutagenesis. Single-factor experiments, the Plackett-Burman technique and response surface methodology were used to optimize the fermentation conditions. This study successfully generated a mutant and innovatively provided important parameters for utilizing BM by fermentation, which could be of industrial value.