کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090078 1081472 2013 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Antibiotic-induced modifications of the stiffness of bacterial membranes
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Antibiotic-induced modifications of the stiffness of bacterial membranes
چکیده انگلیسی

In the latest years the importance of high resolution analysis of the microbial cell surface has been increasingly recognized. Indeed, in order to better understand bacterial physiology and achieve rapid diagnostic and treatment techniques, a thorough investigation of the surface modifications induced on bacteria by different environmental conditions or drugs is essential.Several instruments are nowadays available to observe at high resolution specific properties of microscopic samples. Among these, AFM can routinely study single cells in physiological conditions, measuring the mechanical properties of their membrane at a nanometric scale (force volume). Such analyses, coupled with high resolution investigation of their morphological properties, are increasingly used to characterize the state of single cells.In this work we exploit such technique to characterize bacterial systems. We have performed an analysis of the mechanical properties of bacteria (Escherichia coli) exposed to different conditions. Such measurements were performed on living bacteria, by changing in real-time the liquid environment: standard phosphate buffered saline, antibiotic (ampicillin) in PBS and growth medium. In particular we have focused on the determination of the membrane stiffness modifications induced by these solutions, in particular between stationary and replicating phases and what is the effect of the antibiotic on the bacterial structure.

Figure optionsDownload as PowerPoint slideHighlights
► To understand bacterial physiology, drug-induced surface modifications must be studied.
► We analyzed the mechanical properties of E. coli exposed to different conditions.
► AFM force-volume of the bacteria shows the stiffness modifications caused by ampicillin.
► Stiffer areas are present on the bacterial surface.
► Stiffness tomography probes the stiffness of features lying under the membrane.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 93, Issue 2, May 2013, Pages 80–84
نویسندگان
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