کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2090696 | 1081514 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Selection of high affine peptide ligands for detection of Clostridium Tyrobutyricum spores Selection of high affine peptide ligands for detection of Clostridium Tyrobutyricum spores](/preview/png/2090696.png)
Clostridium tyrobutyricum is the main agent responsible for “late blowing” in cheese, which causes severe economic losses. Nowadays, the reference method for its detection is the Most-Probable-Number (MPN), however, is time consuming and non-specific. Thus, in order to check milk contamination with spores of C. tyrobutyricum, a more specific and rapid method would be required. The objective of this work was to obtain a ligand to establish the basis to develop a biomagnetic separation method for detection of C. tyrobutyricum spores. This study describes the selection of thirteen highly affine peptides to C. tyrobutyricum spores from a phage-display peptide library. In order to test the ability of the peptides attached to a solid support to bind the spores, the most frequent peptide was synthesised and used to coat paramagnetic beads.
Journal: Journal of Microbiological Methods - Volume 79, Issue 2, November 2009, Pages 214–219