کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2090939 1081524 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A sensitive bioassay for the mycotoxin aflatoxin B1, which also responds to the mycotoxins aflatoxin G1 and T-2 toxin, using engineered baker's yeast
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
A sensitive bioassay for the mycotoxin aflatoxin B1, which also responds to the mycotoxins aflatoxin G1 and T-2 toxin, using engineered baker's yeast
چکیده انگلیسی

A novel aflatoxin B1 bioassay was created by introducing a Lipomyces kononenkoae α-amylase gene into a strain of S. cerevisiae capable of expressing the human cytochrome P450 3A4 (CYP3A4), and the cognate human CYP450 reductase. This strain and a dextranase-expressing strain were used in the development of a microtitre plate mycotoxin bioassay, which employed methanol as the solvent and polymyxin B nonapeptide as a permeation enhancer. Stable co-expression of the CYP3A4 gene system and of the dextranase and amylase genes in the two bioassay strains was demonstrated. The bioassay signalled toxicity as inhibition of secreted carbohydrase activity, using sensitive fluorimetric assays. The amylase-expressing strain could detect aflatoxin B1 at 2 ng/ml, and was more sensitive than the dextranase-expressing strain. Aflatoxin G1 could be detected at 2 µg/ml, and the trichothecene mycotoxin T-2 toxin was detectable at 100 ng/ml.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 77, Issue 3, June 2009, Pages 285–291
نویسندگان
, , , ,