کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2091266 | 1081539 | 2006 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Visualization of mcr mRNA in a methanogen by fluorescence in situ hybridization with an oligonucleotide probe and two-pass tyramide signal amplification (two-pass TSA–FISH)
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیوتکنولوژی یا زیستفناوری
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چکیده انگلیسی
Two-pass tyramide signal amplification–fluorescence in situ hybridization (two-pass TSA–FISH) with a horseradish peroxidase (HRP)-labeled oligonucleotide probe was applied to detect prokaryotic mRNA. In this study, mRNA of a key enzyme for methanogenesis, methyl coenzyme M reductase (mcr), in Methanococcus vannielii was targeted. Applicability of mRNA-targeted probes to in situ hybridization was verified by Clone-FISH. It was observed that sensitivity of two-pass TSA–FISH was significantly higher than that of TSA–FISH, which was further increased by the addition of dextran sulphate in TSA working solution. Signals from two-pass TSA–FISH were more reliable compared to the weak, spotty signals yielded by TSA–FISH.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 66, Issue 3, September 2006, Pages 521–528
Journal: Journal of Microbiological Methods - Volume 66, Issue 3, September 2006, Pages 521–528
نویسندگان
Kengo Kubota, Akiyoshi Ohashi, Hiroyuki Imachi, Hideki Harada,