کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2091326 | 1081541 | 2007 | 5 صفحه PDF | دانلود رایگان |
Assessing true numbers of viable anthrax spores is complex. Optimal heat activation conditions vary with species, media and germinants. Published time/temperature combinations for Bacillus anthracis spores range from 60 °C for ≤ 90 min to boiling for 1 min. Results presented here indicate that temperatures are best kept to ≤ 70 °C and holding times need not exceed 15–30 min. Under conditions of 60 °C for 90 min, 62–23 °C for 15 min and 70 °C for 15 or 30 min, although the ratio of heated:unheated counts ranged from < 1 to > 1, post-heating counts were less than their pre-heating counterparts on between 71% and 88% of occasions. A high probability was found of viable spore counts differing significantly from counts determined microscopically, with differences of almost 1 log possible. Viable counts were lower than microscopic counts in 15 of 18 tests.
Journal: Journal of Microbiological Methods - Volume 68, Issue 2, February 2007, Pages 353–357