کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2091373 1081543 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Improved methods for the cultivation of the chemolithoautotrophic bacterium Nitrosomonas europaea
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
Improved methods for the cultivation of the chemolithoautotrophic bacterium Nitrosomonas europaea
چکیده انگلیسی

The chemolithoautotrophic nitrifier Nitrosomonas europaea is useful for biotechnological processes such as bioremediation of toxic chemicals, as well as playing an essential role in the global nitrogen cycle. To successfully apply this bacterium in biotechnology, large quantities of active cells need to be produced. In this paper we demonstrate improved methods for mass cultivation of N. europaea. A doubling in the final cell density to 30 mg dry wt l− 1 was achieved with a fed-batch culture technique using ammonium bicarbonate or sodium bicarbonate for pH control. The cells displayed high ammonia oxidizing activity in excess of 20 mmol g− 1 dry wt h− 1. Continuous culture under energy limited growth conditions (< 0.1 mM residual ammonium) in a bench scale bioreactor using a microfiltration membrane for high cell recycle produced final culture densities greater than 350 mg dry wt l− 1. This system allowed operator controlled culturing of cells under excess substrate as well as energy limited growth conditions with a maximum ammonium oxidation potential in excess of 250 mmol g− 1 dry wt h− 1.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Microbiological Methods - Volume 65, Issue 1, April 2006, Pages 96–106
نویسندگان
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