A fluorometric method for evaluation of pharmacological activity against intracellular Leishmania amastigotes

In this work a simple and novel method to evaluate the efficacy of compounds on intracellular Leishmania amastigotes by using a fluorometric assay has been developed. The new method is sensitive, easy to perform and scalable for high throughput and therefore it could be validated for screening of new anti-leishmanial agents.

► This new fluorometric method is highly sensitive and easy to perform.
► It is suitable for quantification of intracellular viable Leishmania parasites.
► The fluorometric assay is scalable for high throughput.
► It could be validated for screening of new antileishmanial agents.