Application of pyrosequencing for Salmonella enterica rapid identification

Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for hns, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.

► Pyrosequencing identification of Salmonella was studied.
► Six previous Salmonella identification genes were assessed.
► Only invA, iroB and fimY were suitable for Salmonella rapid identification.
► It required less than 3 h to have pyrosequencing identification results.
► Pyosequencing provides another rapid identification method with high specificity.