کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2115292 1084585 2007 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Induction of apoptosis and autophagic cell death by the vanillin derivative 6-bromine-5-hydroxy-4-methoxybenzaldehyde is accompanied by the cleavage of DNA-PKcs and rapid destruction of c-Myc oncoprotein in HepG2 cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی تحقیقات سرطان
پیش نمایش صفحه اول مقاله
Induction of apoptosis and autophagic cell death by the vanillin derivative 6-bromine-5-hydroxy-4-methoxybenzaldehyde is accompanied by the cleavage of DNA-PKcs and rapid destruction of c-Myc oncoprotein in HepG2 cells
چکیده انگلیسی

Autophagy is a regulated lysosomal pathway involving the bulk degradation of cytoplasmic contents, and is an emerging attractive therapeutic approach for treating cancers. In the present study, we demonstrates that bromovanin (6-bromine-5-hydroxy-4-methoxybenzaldehyde), a vanillin derivative, exhibits a potent antiproliferative effect on a broad spectrum of cancer cell lines, but it induces apoptosis with a large variation in extent on different cancer cell lines. Ultrastructural observation in transmission electron microscopy reveals that autophagy is another type of cell death induced by bromovanin in HepG2 cells. Treatment with bromovanin significantly increases cellular ROS level as well as elicits DNA double-strand breaks as indicated by comet assay and the increased phosphorylated H2AX. Cleavage and inactivation of DNA-PKcs induced by bromovanin is found to occur concurrently with a rapid destruction of c-Myc oncoprotein. These multiple effects of bromovanin, especially the induction of both apoptosis and autophagy, make it very appealing for the development as a novel anticancer drug.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 252, Issue 2, 18 July 2007, Pages 280–289
نویسندگان
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