کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2115985 | 1084620 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ex vivo study of MAPK profiles correlated with parameters of apoptosis during cervical carcinogenesis
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کلمات کلیدی
JnkERKPARPHSILc-Jun NH2-terminal kinaseLSILMAPK - MAPKMAPKs - MAPK هاp38-MAPK - p38-mapkApoptosis - خزان یاختهایCervical carcinogenesis - سرطان دهان رحمextracellular signal regulated kinase - سیگنال خارج سلولی kinase را تنظیم می کندHigh grade squamous intraepithelial lesion - ضایعه داخل شکمی مقیاس بزرگlow grade squamous intraepithelial lesion - ضایعه داخلی داخل شکمی ضخیمNormal - طبیعیmitogen activated protein kinase - پروتئین کیناز فعال Mitogen فعال استpoly (ADP-ribose) polymerase - پلی (ADP-ribose) پلیمرازCarcinoma - کارسینوما
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Cervical cancer is a leading cause of death in developing countries and is the second highest occurring cancer in women all over the world. The progression of cancer is a multistep process affecting aspects of cellular function such as proliferation, differentiation and apoptosis. Mitogen activated protein kinases (MAPKs), which include p38-MAPK, c-Jun NH2-terminal kinase (JNK) and extracellular signal-regulated kinases (ERKs) are closely associated with cell proliferation and apoptosis and the balance between them could determine a cell's fate. Despite the expanding research effort in vitro, little is known about MAPK activation in clinical specimens of cervical cancer. Therefore, the aim of this ex vivo study was to correlate the phosphorylation status (activity) of MAPKs (p38-MAPK, JNK and ERK), as well as poly (ADP-ribose) polymerase (PARP) and caspase-3 (two cellular markers of apoptosis), during the different stages of cervical carcinogenesis, to observe whether correlations between MAPK activities and apoptosis during the disease process exist. Decreased p38-MAPK phosphorylation was found in the carcinoma (Ca) group) compared to the normal tissues, as well when the low grade squamous intraepithelial lesion-LSIL) group and high grade squamous intraepithelial lesion-HSIL) group were compared with the Ca group. Interestingly, a significant decrease in ERK44 phosphorylation was observed in Ca when compared to LSIL and HSIL. There was also a significant decrease in JNK phosphorylation in Ca when compared with normal tissue and HSIL. As expected, caspase-3 activation and PARP cleavage was significantly lower in Ca when compared with normal tissue. Our results present the first evidence of in vivo involvement of MAPKs in cervical cancer and indicate a possible correlation between MAPK activities and apoptosis in the disease process.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Cancer Letters - Volume 235, Issue 1, 8 April 2006, Pages 93-99
Journal: Cancer Letters - Volume 235, Issue 1, 8 April 2006, Pages 93-99
نویسندگان
A.-M. Engelbrecht, S. Gebhardt, L. Louw,