کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
21390 | 43220 | 2010 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
N-terminal deletion of Tk1689, a subtilisin-like serine protease from Thermococcus kodakaraensis, copes with its cytotoxicity in Escherichia coli
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Tk1689, a subtilisin-like serine protease from Thermococcus kodakaraensis, was found to be toxic to the host cells when produced in the pro-protein form (Pro-Tk1689) in Eschericia coli. Cytotoxic effect of Pro-Tk1689 was reduced when signal and pro-peptide both were removed and the protein was produced in the mature form (MP-Tk1689). The mature protein was produced in E. coli in the soluble form. Recombinant MP-Tk1689 was catalytically active and exhibited optimum activity at 55 °C and pH 7. Specific activity of the enzyme was 700 U/mg. The enzyme displayed a half life of 80 min at 60 °C.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 4, October 2010, Pages 381–385
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 4, October 2010, Pages 381–385
نویسندگان
Nouman Rasool, Naeem Rashid, Saima Iftikhar, Muhammad Akhtar,