کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2145515 | 1088683 | 2006 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cartilage oligomeric matrix protein is overexpressed by scleroderma dermal fibroblasts
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کلمات کلیدی
SSCCOMPTGFβ-1Scleroderma - اسکلرودرمی systemic sclerosis - اسکلروز سیستمیکtransforming growth factor beta 1 - تبدیل فاکتور رشد بتا 1Dermis - درمیFibroblasts - فیبروبلاست هاFibrosis - فیبروز یا فساد الیافExtracellular matrix - ماتریکس خارج سلولیpolymerase chain reaction. - واکنش زنجیره ای پلیمرازPCR - واکنش زنجیرهٔ پلیمرازCartilage oligomeric matrix protein - پروتئین ماتریکس oligomeric غضروفی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
تحقیقات سرطان
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چکیده انگلیسی
Cartilage oligomeric matrix protein (COMP) is an extracellular glycoprotein that belongs to the thrombospondin gene family. It is found predominantly in cartilage, tendon, ligament, and bone. Mutations in the COMP gene have been linked to the development of pseudoachondroplasia and multiple epiphysial dysplasia. COMP influences the organization of collagen fibrils by interacting with collagens I, II and IX. Gene expression profiling of cultured skin fibroblasts suggested that COMP mRNA levels were elevated in scleroderma. We therefore examined COMP expression in SSc and normal skin biopsies. Immunohistochemistry confirmed that COMP protein accumulates in SSc but not normal skin, with SSc skin showing striking deposition in the papillary and deeper dermis. Significant staining was also seen in non-lesional skin from patients. Due to its involvement in the development of fibrosis, TGFβ was examined for a possible role in regulating COMP expression. Cultured SSc fibroblasts demonstrated greater staining for COMP compared to normal controls prior to stimulation, and TGFβ-1 induced a large increase in mRNA and protein. Murine fibroblasts engineered to overexpress human COMP demonstrated increased levels of fibronectin and collagen in the extracellular matrix. Taken together, these data demonstrate that COMP is overexpressed in SSc skin and cultured fibroblasts possibly due to autocrine TGFβ stimulation, and COMP overexpression is sufficient to stimulate excess matrix deposition. By interactions with other matrix proteins and cells, COMP may play a role in pathogenic matrix deposition.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Matrix Biology - Volume 25, Issue 4, May 2006, Pages 213-222
Journal: Matrix Biology - Volume 25, Issue 4, May 2006, Pages 213-222
نویسندگان
Giuseppina Farina, Raphael Lemaire, Joseph H. Korn, Russell L. Widom,