Simple enrichment and independent cultures to expand bacterial community analysis from gedongsongo hot spring

Denaturing gradient gel electrophoresis was used to identify bacterial communities from Gedongsongo hot spring, Indonesia, by culture-independent and -dependent procedures. Predominant species were found to be closely related to β-proteobacteria, particularly from genus Ralstonia and Delftia. Other species present include α- and γ-proteobacteria and Thermus groups. Some of the strains showed unique 16S rDNA sequences.