کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
21708 | 43236 | 2010 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae Simple metabolite extraction method for metabolic profiling of the solid-state fermentation of Aspergillus oryzae](/preview/png/21708.png)
Solid-state culture of microorganisms is an important style of culture both in the traditional food industry and in the modern fermentation industry. We propose here a simple method for metabolite extraction from the solid-state fermentation of a filamentous fungus, Aspergillus oryzae, which is known as rice-koji. To evaluate the efficiency of metabolite extraction, liquid chromatography–mass spectrometry (LC/MS) was used for simultaneous detection of a wide range of metabolites including amino acids, organic acids and vitamins, which are of interest in rice-koji making. Among eleven different metabolite extraction methods tested, we found that extraction using acetonitrile/water (50:50), coupled with boiling at 70 °C for 5 min, was advantageous in terms of both extraction yield and metabolism quenching. We consider that our method provides a technical basis for metabolome analysis of a solid-state fermentation.
Journal: Journal of Bioscience and Bioengineering - Volume 110, Issue 6, December 2010, Pages 665–669