Development of a glucose biosensor based on electrodeposited gold nanoparticles–polyvinylpyrrolidone–polyaniline nanocomposites

• In-situ decoration of AuNPs into PANI was performed by a one-step process.
• PVP was used as the stabilization and dopant agent.
• A glucose biosensor with good reproducibility was fabricated.

In this work, the fabrication and performances of a glucose biosensor based on the electrodeposited gold nanoparticle–polyvinylpyrrolidone–polyaniline (AuNP–PVP–PANI) nanocomposites have been demonstrated. The nanocomposites were electrodeposited on a glassy carbon electrode (GCE) in a homogeneous three-component solution consisting of aniline, PVP and AuNPs. Glucose oxidase (GOx) was subsequently immobilized on the as-prepared AuNP–PVP–PANI nanocomposites using Nafion as the preventing layer. The electrochemical and electrocatalytic properties of the Nafion/GOx/AuNP–PVP–PANI modified GCE were researched. It exhibited good electrocatalytic performances with a large linear range from 0.05 mM to 2.25 mM, a low detection limit of 1.0 × 10− 5 M (S/N = 3), and a high sensitivity of 9.62 μA mM− 1 cm− 2. This sensor with excellent stability and reproducibility was also allowed for the detection of glucose in human serum samples with satisfactory results.