کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
218351 | 463194 | 2015 | 8 صفحه PDF | دانلود رایگان |
• We have prepared a simple and reproducible biosensor based on DPV and EIS.
• Ease of fabrication, high sensitivity and selectivity are main benefits of designed biosensor.
• Detection limit for target DNA using proposed biosensor was 4.6 × 10−20 M by EIS.
• Measurement of target DNA in the genome samples were done with satisfactory results.
• DNA synthesis was done at the surface of electrode to enhance its selectivity and sensitivity.
In this research, we developed a simple and reproducible electrochemical biosensor based on a gold electrode (GE) to detect DNA. Self-assembled monolayers (SAMs) of thiolated single-stranded DNA (SH-ssDNA), or BRCA1 5382 insC mutation detection (ssDNA), were immobilized on the electrode for a specific time. The immobilization of the probe and its hybridization with the target DNA were optimized using different experimental conditions. The modified electrode was characterized by electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and differential pulse voltammetry (DPV). It was observed that EIS approach had better analytical performance as compared with DPV. The linear range of the biosensor was estimated by EIS and found to be 1.0 × 10−19–1.0 × 10−7 M with a detection limit of 4.6 × 10−20 M. These results demonstrate that the new biosensor can be used to diagnose breast cancer. The biosensor showed excellent selectivity for discriminating complementary sequences from the noncomplementary sequence, so it can be used to detect breast cancer. Measurements of complementary DNA strains in the genome samples were done by EIS with satisfactory results.
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Journal: Journal of Electroanalytical Chemistry - Volume 750, 1 August 2015, Pages 57–64