کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2183745 | 1095587 | 2008 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Generation of multifunctional murine monoclonal antibodies specifically directed to the VP1unique region protein of human parvovirus B19
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کلمات کلیدی
IgGAPLPLA2B19APs - AP هاamino acid - آمینو اسیدMonoclonal antibody - آنتی بادی مونوکلونالAntiphospholipid antibodies - آنتیبادی ضد فسفولیپیدphospholipase A2 - آنزیم فسفولیپاز A2 Arachidonic acid - اسید آراشیدونیکimmunoglobulin G - ایمونوگلوبولین GELISA - تست الیزاEnzyme-linked immunosorbent assay - تست الیزاAntiphospholipid syndrome - سندروم آنتی فسفولیپید wildtype - نوع وحشیHuman parvovirus B19 - پاروویروس انسان B19
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Generation of multifunctional murine monoclonal antibodies specifically directed to the VP1unique region protein of human parvovirus B19 Generation of multifunctional murine monoclonal antibodies specifically directed to the VP1unique region protein of human parvovirus B19](/preview/png/2183745.png)
چکیده انگلیسی
Little is known about the VP1unique region (VP1u), a part of one major capsid protein of human parvovirus B19 (B19), concerning its involvement in viral replication and infection cycle. Showing a phospholipase A2 (PLA2)-like activity, which is discussed to be necessary for viral release from host cell, its precise function remains unclear. The purpose of this study was to generate multifunctional monoclonal antibodies (mabs) for different applications that may be useful in investigating VP1u's relevance. To establish antiVP1u antibodies, spleen cells from Balb/c mice immunized with purified recombinant viral protein were used for generating antibody-producing hybridoma cell lines. Usability of the antibodies was tested in enzyme-linked immunosorbent assay (ELISA), Western-blot analysis, immunofluorescence and an inhibition assay of enzymatic activity of PLA2. Three hybridoma cell lines secreting mab's specifically directed against the VP1u protein of B19 could be generated and functioned in every screening method used in this study. These antibodies are helpful tools for investigations in B19 research and diagnosis. Furthermore, the antibodies could help in gaining a deeper understanding of VP1u's role in viral replication and infection especially in the importance of its constitutive PLA2-like activity.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Immunobiology - Volume 213, Issue 6, 10 July 2008, Pages 511-517
Journal: Immunobiology - Volume 213, Issue 6, 10 July 2008, Pages 511-517
نویسندگان
Maik D. Drechsler, Ingrid Obermeier, Yvonne Döring, Karl J. Lackner, Susanne Modrow, Philipp von Landenberg,