کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2185402 1095978 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Interaction of Capping Protein with the Barbed End of the Actin Filament
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
The Interaction of Capping Protein with the Barbed End of the Actin Filament
چکیده انگلیسی

The interaction of capping protein (CP) with actin filaments is an essential element of actin assembly and actin-based motility in nearly all eukaryotes. The dendritic nucleation model for Arp2/3-based lamellipodial assembly features capping of barbed ends by CP, and the formation of filopodia is proposed to involve inhibition of capping by formins and other proteins. To understand the molecular basis for how CP binds the barbed end of the actin filament, we have used a combination of computational and experimental approaches, primarily involving molecular docking and site-directed mutagenesis. We arrive at a model that supports all of our biochemical data and agrees very well with a cryo-electron microscopy structure of the capped filament. CP interacts with both actin protomers at the barbed end of the filament, and the amphipathic helix at the C-terminus of the β-subunit binds to the hydrophobic cleft on actin, in a manner similar to that of WH2 domains. These studies provide us with new molecular insight into how CP binds to the actin filament.

Graphical AbstractFigure optionsDownload high-quality image (93 K)Download as PowerPoint slideResearch Highlights
► An improved model for the structure of capping protein bound to actin.
► Combination of computational and experimental approaches.
► Consistent with previous cryo-electron microscopy results.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular Biology - Volume 404, Issue 5, 17 December 2010, Pages 794–802
نویسندگان
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