Voltammetric and impedimetric detection of DNA hybridization by using dendrimer modified graphite electrodes

• The surface of graphite electrode (PGE) was modified by PAMAM dendrimer (G4-PDR).
• G4-PDR/PGEs were introduced as the first time for detection of DNA hybridization.
• Voltammetric and impedimetric results were found in a good agreement.

In the present study, the electrochemical sensor was designed by the modification of the fourth generation poly(amido amine) (PAMAM) dendrimer onto the surface of pencil graphite electrode (G4-PDR/PGE) and this single-use dendrimer modified electrode was then examined for advanced nucleic acid detection by using electrochemical techniques. After the covalent immobilization of G4-PDR onto the surface of carboxylated PGE surface, the surface characterization of dendrimer modified PGE was investigated by using scanning electron microscopy (SEM), and also, different electrochemical techniques; cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The voltammetric monitoring of a sequence-selective DNA hybridization related to Hepatitis B virus (HBV) sequences was accordingly performed in the presence of the complementary of probe (target), and also the mixture medium containing target:non-complementary (NC) sequences (1:1).

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