کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2192309 | 1097887 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Control of cardiac myofilament activation and PKC-βII signaling through the actin capping protein, CapZ
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کلمات کلیدی
EGTAEC50C-proteinMyofilamentsPMSFCapZPKCFmaxMOPSPIP2ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid - اتیلن گلیکول بیست (2-aminoethylether) -N، N، N '، N'-tetraacetic acid3-morpholinopropanesulfonic acid - اسید 3-مورفولینو پروپان سولفونیکSDS-PAGE - الکتروفورز ژل پلی آکریل آمیدSodium dodecyl sulfate polyacrylamide gel electrophoresis - الکتروفورز ژل پلی اتیل آمید سدیم دودسیل سولفاتTransgenic - تراریختهphenylmethyl-sulfonyl fluoride - فنیل متیل سولفونیل فلورایدCardiac - قلبProtein kinase C - پروتئین کیناز سی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
Actin capping protein (CapZ) anchors the barbed ends of sarcomeric actin to the Z-disc. Myofilaments from transgenic mice (TG-CapZ) expressing a reduced amount of CapZ demonstrate altered function and protein kinase C (PKC) signaling [Pyle WG, Hart MC, Cooper JA, Sumandea MP, de Tombe PP, and Solaro RJ., Circ. Res. 90 (2002) 1299-306]. The aims of the current study were to determine the direct effects of CapZ on myofilament function and on PKC signaling to the myofilaments. Our studies compared mechanical properties of single myocytes from TG-CapZ mouse hearts to wild-type myocytes from which CapZ was extracted using PIP2. We found that myofilaments from CapZ-deficient transgenic myocardium exhibited increased Ca2+ sensitivity and maximum isometric tension. The extraction of CapZ from wild-type myofilaments replicated the increase in maximum isometric tension, but had no effect on myofilament Ca2+ sensitivity. Immunoblot analysis revealed that the extraction of CapZ was associated with a reduction in myofilament-associated PKC-βII and that CapZ-deficient transgenic myofilaments also lacked PKC-βII. Treatment of wild-type myofilaments with recombinant PKC-βII reduced myofilament Ca2+ sensitivity, whereas this effect was attenuated in myofilaments from TG-CapZ mice. Our results indicate that cardiac CapZ directly controls maximum isometric tension generation, and establish CapZ as an important component in anchoring PKC-βII at the myofilaments, and for mediating the effects of PKC-βII on myofilament function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Molecular and Cellular Cardiology - Volume 41, Issue 3, September 2006, Pages 537-543
Journal: Journal of Molecular and Cellular Cardiology - Volume 41, Issue 3, September 2006, Pages 537-543
نویسندگان
W. Glen Pyle, Gustavo La Rotta, Pieter P. de Tombe, Marius P. Sumandea, R. John Solaro,