کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2198335 1551009 2006 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A generalized numerical approach to rapid-equilibrium enzyme kinetics: Application to 17β-HSD
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A generalized numerical approach to rapid-equilibrium enzyme kinetics: Application to 17β-HSD
چکیده انگلیسی

A generalized numerical treatment of rapid-equilibrium enzyme kinetics is presented. This new approach relies on automatic computer derivation of the underlying mathematical model (a system of simultaneous nonlinear algebraic equations) from a symbolic representation of the reaction mechanism (a system of biochemical equations) provided by the researcher. The method allows experimental biochemists to analyze initial-rate enzyme kinetic data without having to use any mathematical equations. An illustrative example is based on the inhibition kinetics of 17β-hydroxysteroid dehydrogenase type 5 by a class of natural compounds. A computer implementation of the new method, a newly modified software package DYNAFIT [Kuzmič, P., 1996. Program DYNAFIT for the analysis of enzyme kinetic data: application to HIV proteinase. Anal. Biochem. 237, 260–273], is freely available to all academic researchers.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 248, Issues 1–2, 27 March 2006, Pages 172–181
نویسندگان
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