کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2199150 | 1099428 | 2008 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum Activity-dependent volume transmission by transgene NPY attenuates glutamate release and LTP in the subiculum](/preview/png/2199150.png)
Neuropeptide Y (NPY) gene transduction of the brain using viral vectors in epileptogenic regions can effectively suppress seizures in animals, and is being considered as a promising alternative treatment strategy for epilepsy. Therefore, it is fundamental to understand the detailed mechanisms governing the release and action of transgene NPY in neuronal circuitries. Using whole-cell recordings from subicular neurons, we show that in animals transduced by recombinant adeno-associated viral (rAAV) vector carrying the NPY gene, transgene NPY is released during high-frequency activation of CA1-subicular synapses. Released transgene NPY attenuates excitatory synaptic transmission not only in activated, but also in neighboring, non-activated synapses. Such broad action of transgene NPY may prevent recruitment of excitatory synapses in epileptic activity and could play a key role in limiting the spread and generalization of seizures.
Journal: Molecular and Cellular Neuroscience - Volume 39, Issue 2, October 2008, Pages 229–237