کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2201369 | 1100015 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of neuronal and astroglial responses to ER stress in the hippocampal CA1 area in mice following transient forebrain ischemia
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
PAGEBCCAssDNAGRP78PMSFDTTPBSSDSC/EBP homologous protein - C / EBP پروتئین همولوگSingle-stranded DNA - DNA تک رشته ایH&E - H & EAstrocyte - آستروسیتEndoplasmic reticulum stress - استرس شبکه آندوپلاسمیpolyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمیدForebrain ischemia - ایسکمی فروباینCHOP - تکه کردنdithiothreitol - دیتیوتریتولsodium dodecyl sulfate - سدیم دودسیل سولفاتendoplasmic reticulum - شبکه آندوپلاسمی dentate gyrus - شکنج دندانه دارphenylmethane sulfonyl fluoride - فنیل متیل سولفونیل فلورایدPhosphate-buffered saline - محلول نمک فسفات با خاصیت بافریHematoxylin and Eosin - هماتوکسیلین و ائوزینHippocampus - هیپوکامپ 78-kDa glucose-regulated protein - پروتئین تنظیم شده با گلوکز 78 کیلوگرمCaspase-12 - کاسپاز 12
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Transient forebrain ischemia has been shown to cause neuronal injury in the CA1 area of the hippocampus in mice. In addition to neuronal injury, astrocytes in area CA1 undergo apoptosis under ischemic conditions. Although failure of impaired astrocytes to take up glutamate is thought to contribute to the pathogenesis of cerebral ischemia, the molecular mechanism underlying this phenomenon remains unexplored. In the present study, we investigated neuronal and astroglial responses to endoplasmic reticulum (ER) stress, which is an important sequela of transient forebrain ischemia in the hippocampus of mice. Cellular injury was observed in area CA1 of the hippocampus 72Â h after reperfusion, and ssDNA positivity was detectable in some glial cells as well as neurons in this area. An increase of 78-kDa glucose-regulated protein (GRP78), an indicator of ER stress, was detected in pyramidal neurons and astrocytes in this area after the insult. Immunohistochemical analysis showed that caspase-12 was increased in pyramidal neurons and astrocytes located in the extrapyramidal cell layer. Immunoreactivity for C/EBP homologous protein (CHOP) was increased significantly in pyramidal cells but not in astrocytes. These results suggest that astrocytes as well as pyramidal neurons in area CA1 undergo apoptosis through an ER stress-dependent mechanism after ischemia. Unlike the situation in neuronal apoptosis, CHOP does not play a role in the cell death of astrocytes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 57, Issue 1, August 2010, Pages 1-7
Journal: Neurochemistry International - Volume 57, Issue 1, August 2010, Pages 1-7
نویسندگان
Nobuhiro Osada, Yasuhiro Kosuge, Kumiko Ishige, Yoshihisa Ito,