Biosynthesis of copper nanoparticles; its characterization and efficacy against human pathogenic bacterium

• Copper nano particles were prepared by environmentally friendly bio-reduction process from copper salt solution.
• The prepared particles characterized by UV–vis spectroscopy, FTIR and EDS technique confirmed the presence of copper nano-particles. The Z-average diameter of synthesized copper nano-particles was found to be 122.7 nm with polydispersity index (PDI) of 0.116.
• Highly stable copper nano particles with good antibacterial activity were prepared by a sustainable technique for medicinal applications.

Synthesis of nano-material with the controlled size and shape is the prime concern of research in nanotechnology. Recently, biosynthesis of metallic nano-particles has gained popularity owing to its eco-friendliness. Use of natural plant extracts in the preparation of nano-particles provides advancement over chemical and physical methods, due to its t is cost effectiveness and environment friendly nature. In the present work, copper nano-particles have been synthesized with simple and green technique by using Ocimum sanctum plant leaf extract as reducer as well as stabilizer. The size and shape of synthesized nano-particles were pigeonholed by particle size analyzer and SEM, further were characterized by EDS and FT-IR spectral technique for their elemental and chemical composition. The experimental results showed copper nano-particles having Z-average diameter of 122.7 nm with higher stability and significantly higher antibacterial activities.