کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
22205 | 43261 | 2006 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cynomolgus monkey embryonic stem cell lines express green fluorescent protein
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We successfully established cynomolgus monkey embryonic stem (cES) cell lines expressing enhanced green fluorescent protein (GFP) by introducing a GFP-encoding gene under cytomegalovirus immediate early enhancer (CMVIE) promoter regulation into cES cells. The cells maintained the ability of in vitro differentiation toward ectodermal, mesodermal, and endodermal lineages, and produced teratomas composed of tissues derived from the three embryonic germ layers when transplanted into severe combined immunodeficient disease mice. GFP expression was also observed in the differentiated cells. These GFP-expressing cES cell lines are considered useful for basic research, including cell transplantation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 1, July 2006, Pages 14–20
Journal: Journal of Bioscience and Bioengineering - Volume 102, Issue 1, July 2006, Pages 14–20
نویسندگان
Shigehiko Ueda, Masahide Yoshikawa, Yukiteru Ouji, Ko Saito, Kei Moriya, Mariko Nishiofuku, Noriko Hayashi, Shigeaki Ishizaka, Keiji Shimada, Noboru Konishi, Hiroshi Fukui,