کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
225094 464475 2009 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Producing a phenylalanine-free pool of peptides after tailored enzymatic hydrolyses of cheese whey
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Producing a phenylalanine-free pool of peptides after tailored enzymatic hydrolyses of cheese whey
چکیده انگلیسی

Sequential hydrolyses of cheese whey proteins using trypsin, chymotrypsin and carboxypeptidase A (CPA) immobilized on agarose-glyoxyl were studied in this work. Phenylalanine (Phe) was removed from the hydrolysates by the action of CPA, thus providing an adequate source of proteins for phenylketonuria (PKU) patients. The role of the first two pre-hydrolyses on the yield of Phe by CPA was investigated: whey proteins were sequentially hydrolyzed to different degrees of hydrolysis with trypsin and chymotrypsin employing different enzyme/substrate ratios. The analyses indicate that a more suitable substrate for CPA is obtained when only chymotrypsin hydrolyzes whey. Using an ad-hoc approach, an empirical model for the apparent reaction rate was developed, which allows the prediction of the concentration of “hydrolyzed peptide bonds” (P) during the proteolysis with immobilized chymotrypsin. This model fitted the experimental data very well, while the Michaelis–Menten-type equation failed to represent the time-evolution of P in batch assays.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Food Engineering - Volume 91, Issue 1, March 2009, Pages 109–117
نویسندگان
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