کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2421679 | 1552853 | 2014 | 9 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: High-density single nucleotide polymorphisms linkage and quantitative trait locus mapping of the pearl oyster, Pinctada fucata martensii Dunker High-density single nucleotide polymorphisms linkage and quantitative trait locus mapping of the pearl oyster, Pinctada fucata martensii Dunker](/preview/png/2421679.png)
The pearl oyster, Pinctada fucata martensii, is an important aquaculture species in the production of free marine pearls in China and Japan; likewise, it is an ideal animal model for the study of biomineralization. A high-density genetic linkage map was constructed for P. fucata martensii with 3,117 SNP markers. The map spanned 990.74 cM whereas the genome coverage was 99.1% with an average interval of 0.39 cM, a resolution that was the highest in pearl oyster and even higher than that of any other mollusc. QTL mapping and association analysis suggested that six growth-related QTLs were identified, including two QTLs related with total weight and one QTL related with shell length, hinge line length, inner non-nacre covered shell length and shell nacre thickness. One marker, f394, was located at scaffold21710.1|size9744 and 117 bp away from the highly homologous region with intron 2 of N16, a matrix protein gene involved in nacreous layer formation. Taken together, our study generates, for the first time, a high-density genetic linkage map for P. fucata martensii, which enables us to fine-map a gene potentially crucial for pearl formation, thus providing a target gene for selective breeding of P. fucata martensii to improve pearl production.
Journal: Aquaculture - Volume 434, 20 October 2014, Pages 376–384