کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2422572 1552888 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
cDNA cloning and expression profile analysis of an ATP-binding cassette transporter in the hepatopancreas and intestine of shrimp Fenneropenaeus chinensis
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
cDNA cloning and expression profile analysis of an ATP-binding cassette transporter in the hepatopancreas and intestine of shrimp Fenneropenaeus chinensis
چکیده انگلیسی

ATP-binding cassette (ABC) transporters are integral membrane proteins which carry diverse substrates across biological membranes. In this study, we report the cloning of a full-length, 2122-bp cDNA sequence encoding a gene of the ABC transporter subfamily G (ABCG) from the shrimp Fenneropenaeus chinensis. The deduced protein, FcABCG, contains 633 amino acid residues with a theoretical molecular weight of 70.57 kDa. Phylogenetic analysis showed that the FcABCG clusters with the shrimp Litopenaeus vannamei ABCG in the same clade and is more closely related to the insect ABC transporter/white proteins than to the ABCG2 of the fish Salmo salar. High-level mRNA expression of FcABCG was detected in the tissues of hepatopancreas and intestine using a real-time quantitative reverse transcriptase PCR. Furthermore, exposure to alkaline pH stress conditions and norfloxacin diets result in the activation of the FcABCG gene in F. chinensis. These results suggest that the ABCG transporter of F. chinensis might play a role in coping high pH stress and in exporting xenobiotics.


► The first full-length cDNA sequence of ABC transporter in shrimp F. chinensis.
► The FcABCG protein contains 633 amino acid residues with a MW of 70.57 kDa.
► Alkaline pH stress or norfloxacin diet activates FcABCG expression in F. chinensis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volumes 356–357, 1 August 2012, Pages 250–255
نویسندگان
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