کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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2423145 | 1552909 | 2011 | 9 صفحه PDF | دانلود رایگان |
High water temperatures are associated with immune suppression and elevated mortality in economically important marine bivalves. To determine whether the stimulatory effects of β-glucan on enzymes associated with the immune system were depressed at higher temperatures, scallops Chlamys farreri were treated with 0.5, 1.0 or 2.0 mg/ml of β-glucan suspensions at 15 °C or 25 °C, and the activities of six enzymes (phenoloxidase (PO), acid phosphatase (ACP), superoxide dismutase (SOD), peroxidase (POD), myeloperoxidase (MPO) and alkaline phosphatase (ALP)) were measured in haemocytes every 12 h for seven days. The results showed that at 15 °C, compared to the control, the 0.5 mg/ml treatment resulted in significant induction of PO activity, the 1.0 mg/ml treatment stimulated PO, ACP, SOD, POD and MPO activities, and the 2.0 mg/ml treatment stimulated PO, ACP, SOD, POD and ALP activities (p < 0.05). At 25 °C, significant activation of PO activity was observed with the 0.5 mg/ml treatment, while PO, ACP and SOD activities were induced by the 1.0 mg/ml treatment and ACP activity by the 2.0 mg/ml treatment (p < 0.05); however, no significant induction of POD or MPO activity was observed. Surprisingly, ALP activity was undetectable at 25 °C throughout the entire experimental period. These results indicate that the 1.0 mg/ml β-glucan treatment consistently had the highest stimulatory effect among the three concentrations. PO, ACP and SOD were more sensitive than POD, MPO and ALP to β-glucan or temperature stress. The extent and duration of activation as well as the time of peak emergence for all six enzymes appeared higher, longer and earlier at 15 °C than 25 °C in all β-glucan treatments, suggesting that high temperature may weaken or delay immune reactions to exogenous stress.
Journal: Aquaculture - Volume 315, Issues 3–4, 21 May 2011, Pages 213–221