کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2423743 1552929 2010 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential gene expression in Litopenaeus vannamei shrimp in response to diet changes
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم آبزیان
پیش نمایش صفحه اول مقاله
Differential gene expression in Litopenaeus vannamei shrimp in response to diet changes
چکیده انگلیسی

We evaluated changes in gene expression of Litopenaeus vannamei shrimp in response to a change in diet by comparing specimens which were fed with an animal protein diet against those which underwent a change from an animal protein diet to a plant protein diet. In addition, we also compared specimens which were fed with a plant protein diet with another group which underwent a change from plant to animal protein diet. Results showed that, in the case of hepatopancreas tissue, a total of six genes were differentially expressed and exhibited a high degree of homology with sequences which codify for proteins related to metabolism, transcription regulation, and rRNA subunits. In the case of muscle tissue, four differentially expressed sequences were recorded which codified for proteins associated to the immune system response, as well as cell structure and signaling. By means of RT-PCR, we also detected differentially expressed chitinase transcripts (ΔCt). We observed ΔCt expression values of 0.49 when comparing specimens fed a plant diet to those which underwent a change from plant to an animal diet, while a ΔCt value of 0.52 was observed when comparing animals fed with an animal diet to specimens which underwent a change from an animal to a plant protein diet. Differential gene expression was not observed in specimens which did not undergo a change in diet during the experiment.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Aquaculture - Volume 300, Issues 1–4, 27 February 2010, Pages 137–141
نویسندگان
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